Structural roles of lipid molecules in the assembly of plant PSII-LHCII supercomplex

In plants, photosystem II (PSII) associates with light-harvesting complexes II (LHCII) to form PSII-LHCII supercomplexes. They are multi-subunit supramolecular systems embedded in the thylakoid membrane of chloroplast, functioning as energy-converting and water-splitting machinery powered by light energy. The high-resolution structure of a PSII-LHCII supercomplex, previously solved through cryo-electron microscopy, revealed 34 well-defined lipid molecules per monomer of the homodimeric system. Here we characterize the distribution of lipid-binding sites in plant PSII-LHCII supercomplex and summarize their arrangement pattern within and across the membrane. These lipid molecules have crucial roles in stabilizing the oligomerization interfaces of plant PSII dimer and LHCII trimer. Moreover, they also mediate the interactions among PSII core subunits and contribute to the assembly between peripheral antenna complexes and PSII core. The detailed information of lipid-binding sites within PSII-LHCII supercomplex may serve as a framework for future researches on the functional roles of lipids in plant photosynthesis.