Quantitative analytical aspects of reversed-phase liquid chromatography with slurry-packed capillary columns

Conditions for reproducible packing of fused-silica liquid chromatography capillary columns were demonstrated. The plate height vs. velocity curves were determined for successively prepared columns. In addition, the values of separation impedance were calculated. Several liquid chromatography systems were evaluated in conjunction with the slurry-packed capillaries for the retention and peak area reproducibility.