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Cardiac Valve Bioreactor for Physiological Conditioning and Hydrodynamic Performance Assessment
Brandon J Tefft1, Joshua A Choe1, Melissa D Young1
1Department of Cardiovascular Medicine, Mayo Clinic, 200 First St. SW, Rochester, MN, 55905, USA.
Cardiovascular Engineering and Technology
|October 13, 2018
Summary
A new cardiac valve bioreactor system enables conditioning and hydrodynamic assessment of tissue engineered heart valves (TEHV). This system supports various physiological conditions and ensures cell viability for TEHV development.
Area of Science:
- Biomedical Engineering
- Regenerative Medicine
- Cardiovascular Research
Background:
- Current heart valve prostheses have limitations.
- Tissue engineered heart valves (TEHV) offer a promising alternative.
- Standardized conditioning and assessment methods for TEHV are needed.
Purpose of the Study:
- To develop a cardiac valve bioreactor system for TEHV.
- The system should condition living valves under diverse hydrodynamic conditions.
- It must also assess hydrodynamic performance according to ISO 5840 standards.
Main Methods:
- A Windkessel-based bioreactor system was designed.
- Key features include a specialized valve chamber and pressure feedback control.
- System validation involved testing with a bioprosthesis and cell cultures.
Main Results:
- The bioreactor generated a spectrum of hydrodynamic conditions, from static to hypertensive.
- The system maintained asepsis for 4 weeks and cell viability for 2 weeks.
- It recorded data for calculating effective orifice area and regurgitant fraction.
Conclusions:
- A versatile bioreactor system for TEHV has been developed.
- This system facilitates tailored conditioning protocols for unique TEHV designs.
- It enables comprehensive hydrodynamic performance assessment for TEHV.
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