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Updated: Feb 4, 2026

Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain
Published on: March 17, 2023
Rapid and Efficient Genetic Transformation of Sorghum via Agrobacterium-Mediated Method
Phat Tien Do1,2, Hyeyoung Lee3, Kimberly Nelson-Vasilchik4
1Christopher S. Bond Life Sciences Center, Division of Plant Sciences, University of Missouri, Columbia, Missouri.
Abstract:
Genetic transformation via Agrobacterium-mediated methodology has been used in many sorghum studies. However, the transformation efficiency still varies significantly due to high dependence on sorghum genotypes and technical expertise. In this article, we describe a sorghum transformation procedure in sufficient detail using a public genotype, P898012. This system utilizes a standard binary transgenic vector carrying the bar gene as a selectable marker and immature embryos as starting explants. Glufosinate is employed as the selective agent during callus and shoot induction. This procedure is relatively rapid, efficient, highly reproducible, and should be applicable for many other sorghum genotypes. © 2018 by John Wiley & Sons, Inc.
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