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Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration
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The involvement of regulated in development and DNA damage response 1 (REDD1) in the pathogenesis of intervertebral

Huipeng Yin1, Yukun Zhang1, Kun Wang1

  • 1Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

Experimental Cell Research
|October 13, 2018
PubMed

Insights

Regulated in development and DNA damage response 1 (REDD1) protein is elevated in intervertebral disc degeneration (IDD). REDD1 contributes to extracellular matrix damage, offering a potential therapeutic target for IDD.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Pathology

Background:

  • Regulated in development and DNA damage response 1 (REDD1) is a stress-responsive protein implicated in various diseases.
  • Intervertebral disc degeneration (IDD) is a debilitating condition with complex underlying mechanisms.

Purpose of the Study:

  • To investigate the role of REDD1 in the pathogenesis of intervertebral disc degeneration (IDD).

Main Methods:

  • Analysis of clinical tissue samples from IDD patients.
  • Overexpression and knockdown studies in human nucleus pulposus (NP) cells.
  • In vitro IDD models under normoxia and hypoxia.
  • Assessment of extracellular matrix (ECM) degeneration, apoptosis, and autophagy.
  • Investigation of the NF-κB signaling pathway.

Main Results:

  • REDD1 expression is upregulated in IDD and correlates with disease severity.
  • REDD1 overexpression induces ECM degeneration in NP cells.
  • Hypoxia downregulates REDD1 via NF-κB, but REDD1 knockdown rescues ECM.
  • The NF-κB/REDD1 pathway mediates protection against apoptosis and autophagy in NP cells.

Conclusions:

  • REDD1 plays a significant role in IDD pathogenesis.
  • Targeting REDD1 presents a potential therapeutic strategy for IDD treatment.
Keywords:
HypoxiaIntervertebral disc degenerationREDD1ROSSerum deprivation

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