Surfactant enhanced flow injection chemiluminescence method for vitamin D₃ determination in pharmaceutical...

A flow injection based chemiluminescence process has been reported for vitamin D₃ determination without using especial chemiluminescence reagent. Vitamin D₃ shows enhancement on the CL intensity of diperiodatocuprate(III) with surfactant (Triton X-100) solution. The calibration curve was found to be linear over the concentration range 0.01-40 mg/L (R² = 0.9997, n = 8) tested. A limit of detection (S/N = 3), limit of quantification (S/N = 10) and sample injection throughput of 2.5 × 10^-3, 8.3 × 10^-3 mg/L and 150 h^-1 respectively were obtained. Various experimental variables were tested to get most suitable response, e.g., the concentrations of reagents, and their flow rates, sample injection volume and photomultiplier tube voltage. The effect of potential interferences was also examined. Vitamin D₃ determination was successfully carried out in pharmaceutical formulations. The recoveries from the formulations were obtained in the range of 96 ± 4-108 ± 2%. The reaction mechanism discussion for diperiodatocuprate(III) complex-Triton X-100-vitamin D₃ was also included.