Related Experiment Video
Updated: Aug 8, 2026

Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance
Published on: July 22, 2011
[Resistance of the suppressor function of T-cells to agents possessing an antiproliferative action]
Abstract:
Administration of cyclophosphamide in a dose of 50 to 400 mg/kg to mice immunized with sheep red blood cells failed to decrease significantly the capacity of the splenic cells of these mice to suppress the primary immune response in transplantation to intact syngeneic recipients. Irradiation of the donors of immune splenic cells (ISC) in a dose of 900 r or treatment of ISC in vitro with mitomycin C failed to influence their suppressor activity. Supernatant obtained after the ultracentrifugation of ISC treated with ultrasound inhibited the primary immune response of intact mice. A conclusion was drawn that the suppressor effect of ISC was caused by the factor produced by T-cells. Active proliferation of these cells was not necessary for the realization of its action.
More Related Videos
09:57Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells
Published on: January 29, 2019
10:21In Vitro Functional Analysis of Regulatory T cells: Focus On Proliferation And Differentiation
Published on: June 9, 2026
Related Concept Videos
Cell-mediated Immune Responses
Abnormal Proliferation
Loss of Tumor Suppressor Gene Functions
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
Cancer-Critical Genes II: Tumor Suppressor Genes
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
T Cell Types and Functions
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Cytotoxic T Cells-mediated Immune Response
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...