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Bacteriophages associated with multiresistant Staphylococcus aureus in Australia.

D M Wilkinson, S Andrews, P R Stewart

    Journal of Medical Microbiology
    |March 1, 1987
    PubMed
    Summary

    Multiresistant Staphylococcus aureus strains from Australian hospitals were analyzed for bacteriophage. All 19 strains contained type 2 DNA, indicating its widespread presence in these bacteria.

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    Area of Science:

    • Microbiology
    • Virology
    • Bacteriology

    Background:

    • Multiresistant Staphylococcus aureus poses a significant threat in hospital settings.
    • Lysogenic bacteriophages can influence bacterial virulence and antibiotic resistance.
    • Understanding phage carriage in S. aureus is crucial for infection control.

    Purpose of the Study:

    • To investigate the presence and characteristics of lysogenic bacteriophages in multiresistant Staphylococcus aureus strains from Australian hospitals.
    • To determine the genetic relatedness of induced phages and their DNA.
    • To identify the prevalence of specific phage DNA sequences in these strains.

    Main Methods:

    • Induction of prophages using mitomycin C and ultraviolet irradiation.
    • Phage typing and characterization of induced phages (type 1 and type 2).

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  • Analysis of phage DNA using endonuclease digestion, hybridization, and Southern blotting.

    • Main Results:

    • Thirteen of 19 strains yielded inducible prophages (type 1 and type 2).
    • Type 1 phage plated on specific S. aureus propagating strains, while type 2 plated only on strain 6.
    • All 19 strains contained type 2 DNA, detected by Southern blotting, suggesting its presence in a defective prophage state.

    Conclusions:

    • Type 2 DNA is universally present in the examined multiresistant Staphylococcus aureus strains from Australian hospitals.
    • The widespread presence of type 2 DNA suggests a potential role in the characteristics of these bacterial strains.
    • Further research is warranted to elucidate the functional significance of type 2 DNA in S. aureus.