The immunological activities of components isolated from Corynebacterium parvum in mice injected with polyoma tumor...

The inhibition of polyoma tumor in CBA mice after immunization with different fractions of Corynebacterium parvum was investigated. Treatment with 200 micrograms of polysaccharide from culture filtrate before s.c. inoculation 2 X 10(6) of tumor cells induced antitumor effect in mice. Treatment with lower doses (1 microgram or 20 micrograms) after transplantation of tumor cells was not effective. Injection of lipopolysaccharide from Escherichia coli abolished a positive response on inhibition of polyoma tumor growth after immunization with Corynebacterium parvum fractions. Additionally, delayed-type hypersensitivity reaction to protein fractions contain tumor antigenic components of membrane vesicles from polyoma tumor cells in the mice immunized with different compounds of Corynebacterium parvum was very strong. These findings suggest that the Corynebacterium parvum preparations are capable to inhibit tumor growth and could be used in special circumstances in immunoprophylaxis.