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Updated: Feb 4, 2026
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Body Composition and Metabolic Caging Analysis in High Fat Fed Mice
Published on: May 24, 2018
16.4K
Lipectomizing Mice for Applications in Metabolism.
Debrup Chakraborty1, Jamie J Bernard2
1Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI, USA.
Methods in Molecular Biology (Clifton, N.J.)
|October 14, 2018
Summary
This study details a surgical method for removing mouse gonadal fat pads. This technique aids research into how visceral adipose tissue (VAT) impacts metabolic health and inflammation.
Area of Science:
- Endocrinology
- Immunology
- Metabolic disease research
Background:
- Obesity is a major public health issue linked to metabolic disorders.
- White adipose tissue, particularly visceral adipose tissue (VAT), plays a key role in regulating metabolism.
- VAT contributes to chronic inflammation and insulin resistance by accumulating immune cells and producing cytokines.
Purpose of the Study:
- To describe a technical protocol for the surgical removal of gonadal fat pads in mice.
- To facilitate research into the metabolic, endocrine, and immunological functions of VAT.
Main Methods:
- Surgical lipectomy procedure focused on gonadal fat pads in mice.
- Detailed technical steps for efficient and successful fat pad removal.
Main Results:
- Successful execution of the lipectomy protocol.
- Provides a reproducible method for VAT removal in mice.
Conclusions:
- The described protocol enables experimental investigation of VAT's role in metabolic disease.
- Facilitates further understanding of obesity-related health issues through VAT manipulation.
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