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Body Composition and Metabolic Caging Analysis in High Fat Fed Mice
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Lipectomizing Mice for Applications in Metabolism.

Debrup Chakraborty1, Jamie J Bernard2

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This study details a surgical method for removing mouse gonadal fat pads. This technique aids research into how visceral adipose tissue (VAT) impacts metabolic health and inflammation.

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Area of Science:

  • Endocrinology
  • Immunology
  • Metabolic disease research

Background:

  • Obesity is a major public health issue linked to metabolic disorders.
  • White adipose tissue, particularly visceral adipose tissue (VAT), plays a key role in regulating metabolism.
  • VAT contributes to chronic inflammation and insulin resistance by accumulating immune cells and producing cytokines.

Purpose of the Study:

  • To describe a technical protocol for the surgical removal of gonadal fat pads in mice.
  • To facilitate research into the metabolic, endocrine, and immunological functions of VAT.

Main Methods:

  • Surgical lipectomy procedure focused on gonadal fat pads in mice.
  • Detailed technical steps for efficient and successful fat pad removal.

Main Results:

  • Successful execution of the lipectomy protocol.
  • Provides a reproducible method for VAT removal in mice.

Conclusions:

  • The described protocol enables experimental investigation of VAT's role in metabolic disease.
  • Facilitates further understanding of obesity-related health issues through VAT manipulation.