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Sperm quality during extra-gonadal sperm reserve depletion in stallions

Jose A Len1, David Beehan1, Bruce Eilts1

  • 1Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana.

Reproduction in Domestic Animals = Zuchthygiene
|October 14, 2018
PubMed

Insights

Sperm quality in stallions peaks on day 3 of extra-gonadal reserves (EGR) depletion. Daily collection for 7 days showed improved plasma membrane integrity by day 3, with motility and morphology remaining consistent.

Area of Science:

  • Animal Science
  • Reproductive Biology
  • Veterinary Medicine

Background:

  • Understanding stallion reproductive potential is crucial for breeding programs.
  • Extra-gonadal reserves (EGR) represent a stallion's sperm reserves.
  • Optimizing sperm collection timing can maximize fertility outcomes.

Purpose of the Study:

  • To determine the optimal time for sperm collection during EGR depletion to achieve highest sperm quality in stallions.
  • To evaluate changes in sperm parameters throughout the EGR depletion period.

Main Methods:

  • Six light breed stallions were subjected to daily semen collection for 7 days to deplete EGR.
  • Sperm output, total motility (TM), progressive motility (PM), morphology, and plasma membrane (PLM) integrity were assessed on collection days 1, 3, 5, and 7.

Main Results:

  • Sperm output decreased and stabilized by days 5-7 of EGR depletion.
  • Total motility (TM), progressive motility (PM), and sperm morphology did not significantly change during EGR depletion.
  • Plasma membrane integrity (PLM) improved from day 1 to day 3, with no further significant changes observed on days 5 and 7.

Conclusions:

  • The highest sperm quality in sexually rested stallions is achieved on day 3 of the EGR depletion period.
  • Daily collection protocols can be adjusted based on EGR depletion to optimize sperm quality for artificial insemination or cryopreservation.

Keywords:
EGR and sperm outputplasma membranespermstallion

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