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Updated: Feb 4, 2026

Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic
Published on: September 4, 2012
Methods for Diagnosing Mastitis
Pamela R F Adkins1, John R Middleton1
1Department of Veterinary Medicine and Surgery, University of Missouri, 900 East Campus Drive, Columbia, MO 65211, USA.
Abstract:
A diagnosis of mastitis is based on clinical observations or direct/indirect measures of the inflammatory response to infection, whereas a diagnosis of an intramammary infection is based on identification of the infectious agent. Somatic cell count/somatic cell score are common diagnostic tests for the detection of subclinical mastitis. Culture and polymerase chain reaction can be useful in the diagnosis of an intramammary infection; however, both have their advantages and disadvantages. Diagnosing the bacterial agent causing the intramammary infection can help to determine treatment and prevention strategies on the farm, which in turn can help to reduce incidence and prevalence.
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