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Spatial Separation of Molecular Conformers and Clusters
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Two-dimensional insertable separation tool (TWIST) for flow confinement in spatial separations.

Theodora Adamopoulou1, Sander Deridder2, Gert Desmet2

  • 1Van't Hoff Institute for Molecular Sciences (HIMS), Faculty of Science, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, the Netherlands.

Journal of Chromatography. A
|October 15, 2018
PubMed
Summary
This summary is machine-generated.

We developed the TWIST concept, a novel device for spatial comprehensive two-dimensional liquid chromatography (xLCxLC). TWIST ensures precise analyte control for enhanced separation efficiency and shorter analysis times.

Keywords:
3D-printingComputational fluid dynamicsFlow confinementMicrofluidicsMulti-dimensional separationsSpatial liquid chromatography

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Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • Spatial comprehensive two-dimensional liquid chromatography (xLCxLC) offers high peak capacities.
  • Effective flow control and analyte confinement are crucial for xLCxLC performance.

Purpose of the Study:

  • To introduce the TWIST (TWo-dimensional Insertable Separation Tool) concept for improved xLCxLC.
  • To address challenges in flow control and analyte management in xLCxLC.

Main Methods:

  • Designed a modular TWIST device with a rotatable, cylindrical first-dimension (1D) part containing through-holes.
  • Integrated the 1D part within an external casing featuring a flow distributor and second-dimension (2D) channels.
  • Utilized rotation of the 1D part to control analyte confinement and enable transversal flow for 2D injection.

Main Results:

  • The TWIST concept allows for precise confinement of analytes within the 1D channel during initial separation.
  • Rotation of the 1D part facilitates seamless transfer of analytes to the 2D separation stage.
  • Achieved homogeneous flow in the second dimension through controlled mobile phase transfer.

Conclusions:

  • The TWIST device effectively manages flow and analyte positioning in xLCxLC.
  • This modular tool enhances the potential of xLCxLC for high-efficiency separations.
  • TWIST offers a promising solution for optimizing complex chromatographic analyses.