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Related Experiment Video

Updated: Feb 4, 2026

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
10:01

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase

Published on: December 4, 2017

12.7K

Hydrogenases.

Nathaniel S Sickerman1, Yilin Hu2

  • 1Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 15, 2018
PubMed
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Hydrogenases are crucial enzymes converting hydrogen gas to protons and electrons. This chapter details their diverse types, functions, and catalytic mechanisms in various organisms.

Area of Science:

  • Biochemistry
  • Enzymology
  • Microbiology

Background:

  • Hydrogenases are metalloenzymes catalyzing the reversible conversion of H2 to protons and electrons.
  • These enzymes are vital for redox and signaling pathways in prokaryotes, lower eukaryotes, and archaea.
  • Hydrogenases exist in diverse forms, classified into [NiFe], [FeFe], and [Fe] variants based on active site metal composition.

Purpose of the Study:

  • To provide a comprehensive overview of hydrogenase diversity.
  • To discuss the catalytic mechanisms of different hydrogenase types.
  • To summarize biochemical and spectroscopic methods used in hydrogenase research.

Main Methods:

  • Biochemical assays
  • Spectroscopic techniques
Keywords:
Fe-GP cofactorH-clusterHydrogenase[FeFe] hydrogenase[Fe] hydrogenase[NiFe] hydrogenase

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  • Bioinformatic analysis

    • Main Results:

    • Detailed classification of hydrogenases into [NiFe], [FeFe], and [Fe] groups.
    • Elucidation of distinct catalytic mechanisms for each hydrogenase class.
    • Identification of diverse biological roles across various life forms.

    Conclusions:

    • Hydrogenases represent a functionally and structurally diverse enzyme class essential for microbial metabolism.
    • Understanding hydrogenase mechanisms and diversity is key to harnessing their biotechnological potential.
    • Ongoing research utilizes advanced biochemical and spectroscopic methods to further explore these metalloenzymes.