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Updated: Feb 3, 2026

Visualization of Germinosomes and the Inner Membrane in Bacillus subtilis Spores
Published on: April 15, 2019
New Biocide Foam Containing Hydrogen Peroxide for the Decontamination of Vertical Surface Contaminated With Bacillus
Esther Le Toquin1,2, Sylvain Faure3, Nicole Orange2
1Laboratoire Innovations technologiques pour la Détection et le Diagnostic, Service de Pharmacologie et Immunoanalyse, DRF, CEA, INRA, Bagnols-sur-Cèze, France.
Abstract:
Despite scientific advances, bacterial spores remain a major preoccupation in many different fields, such as the hospital, food, and CBRN-E Defense sector. Although many disinfectant technologies exist, there is a lack for the decontamination of difficult to access areas, outdoor sites, or large interior volumes. This study evaluates the decontamination efficiency of an aqueous foam containing hydrogen peroxide, with the efficiency of disinfectant in the liquid form on vertical surfaces contaminated by Bacillus thurengiensis spores. The decontamination efficiency impact of the surfactant and stabilizer agents in the foam and liquid forms was evaluated. No interferences were observed with these two chemical additives. Our results indicate that the decontamination kinetics of both foam and liquid forms are similar. In addition, while the foam form was as efficient as the liquid solution at 4°C, it was even more so at 30°C. The foam decontamination reaction follows the Arrhenius law, which enables the decontamination kinetic to be predicted with the temperature. Moreover, the foam process used via spraying or filling is more attractive due to the generation of lower quantity of liquid effluents. Our findings highlight the greater suitability of foam to decontaminate difficult to access and high volume facilities compared to liquid solutions.
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