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Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer SALB Method
Published on: December 1, 2015
Response surface optimization of lipid and protein extractions from Spirulina platensis using ultrasound assisted
H Hadiyanto1,2, Nais Pinta Adetya1
11Department of Chemical Engineering, Faculty of Engineering, Diponegoro University, Jl. Prof Soedarto, SH Tembalang, Semarang, 50275 Indonesia.
Abstract:
In this study, we optimized the process for extracting lipids and proteins from wet biomasses of Spirulina sp. using a 4-kHz ultrasonic osmotic shock method with ultrasound enhancement at a constant frequency of 40 kHz. Optimization was conducted using a response surface methodology (RSM) at an osmotic NaCl concentration of 10-30%, solvent:biomass ratio of 5-15 v/w, and extraction times of 20-50 min. The present osmotic shock method with ultrasound irradiation increased lipid yields to 6.65% in the presence of 11.9% NaCl, a solvent:biomass ratio of 12:1 v/w, and a 22-min extraction time, and protein yields to 43.96% with 15.12% NaCl, a solvent:biomass ratio of 10:1 v/w, and a 30-min extraction time.
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