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Updated: Feb 3, 2026

Murine Model of Wound Healing
05:39

Murine Model of Wound Healing

Published on: May 28, 2013

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An effective range of polydeoxyribonucleotides is critical for wound healing quality

Kyu-Hee Hwang1, Ji-Hee Kim1, Eun Young Park2

  • 1Department of Physiology, Yonsei University Wonju College of Medicine, Wonju, Gangwon‑do 26426, Republic of Korea.

Molecular Medicine Reports
|October 16, 2018
PubMed

Insights

The study found that classic Polydeoxyribonucleotide (PDRN), with a molecular weight of 50–1,500 kDa, optimally enhances wound healing quality by increasing collagen and reducing lipids. This PDRN also boosts cell migration, suggesting a new therapeutic strategy for tissue regeneration.

Area of Science:

  • Biomedical Science
  • Regenerative Medicine
  • Dermatology

Background:

  • Wound healing is a complex biological process involving cellular events and extracellular matrix alterations.
  • Polydeoxyribonucleotide (PDRN), derived from fish sperm, is known to promote tissue regeneration.
  • The optimal molecular weight of PDRN for wound healing remains undetermined.

Purpose of the Study:

  • To investigate the effect of different molecular weights of PDRN on skin wound healing quality.
  • To determine the most effective PDRN molecular size for improving tissue regeneration.
  • To explore PDRN's impact on cellular behavior and signaling pathways in wound healing.

Main Methods:

  • Utilized a mouse skin wound healing model with low (<50 kDa), middle (50–1,500 kDa), and high (>1,500 kDa) molecular weight PDRNs.
  • Assessed regeneration quality using hematoxylin and eosin and Masson's trichrome staining.
  • Evaluated PDRN-mediated cellular behavior via in vitro scratch assays and signaling pathways through western blot analysis.

Main Results:

  • No significant difference in apparent surface wound healing was observed across different PDRN molecular sizes.
  • Immunohistochemical analysis showed reduced lipid accumulation and increased collagen composition in mice treated with classic PDRN (50–1,500 kDa).
  • Classic PDRN enhanced human fibroblast cell migration through c-Jun N-terminal kinase signaling.

Conclusions:

  • The 50–1,500 kDa molecular weight range of PDRN is optimal for improving wound healing quality.
  • Classic PDRN demonstrates potential as a therapeutic agent to enhance tissue regeneration.
  • This study provides insights into optimal PDRN molecular weight for wound healing and novel therapeutic strategies.

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