Comparative transcriptome analysis explores maternal to zygotic transition during Eriocheir sinensis early
Junhao Ning1, Chengwen Song2, Danli Luo1
1CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Gene
|October 16, 2018
Summary
Early animal development relies on maternal genes until the maternal to zygotic transition (MZT). This study reveals MZT occurs during cleavage in Eriocheir sinensis, with key gene regulation for development.
Area of Science:
- Developmental Biology
- Genomics
- Molecular Biology
Background:
- Maternal genome directs early animal development.
- The maternal to zygotic transition (MZT) involves maternal gene clearance and zygotic genome activation (ZGA).
Purpose of the Study:
- Investigate molecular mechanisms of MZT in Eriocheir sinensis.
- Characterize transcriptome dynamics during early embryonic development.
Main Methods:
- RNA-Seq technology applied to fertilized eggs and early embryos (cleavage and blastula stages).
- Analysis of 32,088 annotated unigenes, identifying maternal and zygotic genes.
- Identification of conserved maternal (COMATs) and zygotic transcripts (COZYTs).
Main Results:
- Isolated 566 maternal and 1165 zygotic genes, including 103 COMATs and 266 COZYTs.
- COMATs exhibit housekeeping functions essential for Bilateria embryogenesis.
- Identified differentially expressed genes related to MZT, morphogenesis, and immunity, pinpointing MZT around the cleavage stage.
Conclusions:
- MZT in Eriocheir sinensis occurs during the cleavage stage.
- Datasets provide a resource for understanding early development and asynchronous development in crabs.
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