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Spin trapping artifacts in DMSO.

K Stolze, R P Mason

    Biochemical and Biophysical Research Communications
    |March 30, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Dimethyl sulfoxide (DMSO) in alkaline conditions decomposes to form a stable sulfur trioxide anion radical. This finding corrects previous misidentification of this radical adduct as superoxide.

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    Area of Science:

    • Analytical Chemistry
    • Free Radical Chemistry
    • Biochemistry

    Background:

    • Spin trapping is a technique used to detect short-lived radical species.
    • Dimethyl sulfoxide (DMSO) is a common solvent used in various chemical and biological applications.
    • Previous studies have reported the spin adduct of superoxide radical under specific conditions.

    Purpose of the Study:

    • To investigate the nature of radical adducts formed during spin-trapping experiments in alkaline DMSO solutions.
    • To identify the specific radical species generated from DMSO decomposition under alkaline conditions.
    • To clarify potential misassignments of radical adducts in prior research.

    Main Methods:

    • Spin-trapping experiments were conducted using sodium 3,5-dibromo-4-nitrosobenzenesulfonate (DBNBS) as the spin trap.

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  • Alkaline aqueous dimethyl sulfoxide (DMSO) solutions were employed.
  • Comparison was made with radical adducts generated from sulfite oxidation using horseradish peroxidase and hydrogen peroxide.

    • Main Results:

    • A stable sulfur trioxide anion radical adduct was consistently detected in alkaline DMSO solutions.
    • The observed adduct was identical to that formed from sulfite oxidation, indicating a common radical species.
    • The stability of the adduct allowed for the detection of satellite peaks from natural abundance 13C and 33S isotopes.

    Conclusions:

    • Under alkaline conditions, DMSO decomposes in the presence of air to generate the sulfur trioxide anion radical.
    • The previously reported stable spin adduct of superoxide was likely this DMSO-derived sulfur trioxide anion radical.
    • This study corrects a misassignment in the literature regarding the identity of a stable spin adduct.