Related Experiment Video

Updated: Feb 3, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

5.8K

Complete Genome Sequence of the Model Halovirus PhiH1 (ΦH1)

Mike Dyall-Smith1,2, Felicitas Pfeifer3, Angela Witte4

  • 1Computational Biology Group, Max-Planck-Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany. mike.dyallsmith@gmail.com.

Genes
|October 17, 2018
PubMed

Insights

The complete genome of the halophilic myohalovirus Halobacterium virus phiH (ΦH) was sequenced, revealing a 58,072 bp dsDNA genome with 97 protein-coding genes. This provides crucial data for comparative genomics and understanding virus diversity.

Area of Science:

  • Virology
  • Genomics
  • Microbiology

Background:

  • Halobacterium virus phiH (ΦH), a halophilic myohalovirus, was discovered in 1982 and extensively used as a model for haloarchaea genetics.
  • Previous studies had only partially sequenced the ΦH viral genome.

Purpose of the Study:

  • To determine the complete genome sequence of the major variant (phiH1) of Halobacterium virus phiH (ΦH).
  • To integrate genomic data with existing transcription mapping for a comprehensive understanding of the virus.

Main Methods:

  • Sanger sequencing combined with high-coverage Illumina sequencing was employed.
  • Bioinformatic analysis was used to identify protein-coding genes and compare with existing transcription data.

Main Results:

  • The complete dsDNA genome of phiH1 is 58,072 bp and encodes 97 protein-coding genes.
  • ΦH was classified into Myoviridae Type1, Cluster 4 based on structural proteins (VIRFAM).
  • The closest relative identified was Natrialba virus phiCh1 (φCh1), sharing 63% nucleotide identity and significant gene synteny.

Conclusions:

  • The complete genome sequence of ΦH provides a valuable resource for comparative genomics.
  • This finding enhances our understanding of halovirus diversity and evolution.
  • The study facilitates future research into the molecular genetics and diversity of haloarchaeal viruses.
Keywords:
ArchaeaHalobacterium salinarumgenome inversionhaloarchaeahalobacteriahalophagehalovirusvirus

More Related Videos

Ultra-long Read Sequencing for Whole Genomic DNA Analysis10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

24.0K
Novel Sequence Discovery by Subtractive Genomics09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

9.1K

Related Concept Videos

Genomics02:02

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.7K
Genomic Imprinting and Inheritance02:30

Genomic Imprinting and Inheritance

Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
37.2K
Per-Unit Sequence Models01:26

Per-Unit Sequence Models

An ideal Y-Y transformer, grounded through neutral impedances, displays per-unit sequence networks akin to those of a single-phase ideal transformer when subjected to balanced positive- or negative-sequence currents. These currents do not produce neutral currents, and their associated voltage drops.
Zero-sequence currents, which are identical in magnitude and phase, generate a neutral current, resulting in voltage drops across the neutral impedance and the low-voltage winding. If the...
450
Genome Size and the Evolution of New Genes03:21

Genome Size and the Evolution of New Genes

While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
9.1K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.8K
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes02:16

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
16.1K