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Comparative genomics of phenotypic antimicrobial resistances in methicillin-resistant Staphylococcus pseudintermedius

Alice Wegener1, Els M Broens1, Aldert Zomer1

  • 1Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, the Netherlands.

Veterinary Microbiology
|October 17, 2018
PubMed

Insights

Genomic analysis of methicillin-resistant Staphylococcus pseudintermedius (MRSP) in dogs reveals that antimicrobial resistance genotypes accurately predict phenotypes. Clonal complexes correlate with specific resistance gene patterns, aiding in understanding MRSP evolution.

Area of Science:

  • Veterinary Microbiology
  • Genomics
  • Antimicrobial Resistance

Background:

  • Staphylococcus pseudintermedius is a significant canine pathogen.
  • Methicillin-resistant S. pseudintermedius (MRSP), often multidrug-resistant, has been prevalent in Dutch dogs since 2004.

Purpose of the Study:

  • To correlate canine MRSP resistance phenotypes with genotypic antimicrobial resistance markers.
  • To investigate MRSP phylogeny through genomic comparisons.

Main Methods:

  • Whole-genome sequencing of fifty clinical MRSP isolates from dogs.
  • Identification and association of resistance genes with phenotypic resistance for 21 antimicrobials.
  • Single-nucleotide polymorphism (SNP) based phylogeny construction.

Main Results:

  • 98.3% of phenotypic resistances were explained by resistance genes or mutations.
  • Discrepancies in resistance were often due to gene mutations (43.8%).
  • Resistance gene content varied significantly across different MRSP clonal complexes (CC45, CC71, CC258).

Conclusions:

  • Antimicrobial genotypes from whole-genome sequencing are highly predictive of MRSP resistance phenotypes.
  • MRSP clonal complexes are linked to distinct antimicrobial resistance gene patterns.
Keywords:
Comparative genomicsMICMultidrug resistancePhenotypeStaphylococcus pseudintermedius

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