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Long-noncoding RNA IFNG-AS1 exerts oncogenic properties by interacting with epithelial splicing regulatory protein 2

Guohui Lu1, Jian Duan1, Dongwei Zhou1

  • 1Department of Neurosurgery, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China.

Pathology, Research and Practice
|October 17, 2018
PubMed

Insights

Pituitary adenoma (PA) is a challenging cancer. This study reveals that IFNG-AS1 acts as an oncogene in PA by interacting with ESRP2, suggesting new therapeutic targets for pituitary adenoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Endocrinology

Background:

  • Pituitary adenoma (PA) remains a difficult-to-treat cancer despite medical advancements.
  • Understanding the molecular underpinnings of PA is crucial for developing novel therapeutic strategies.

Purpose of the Study:

  • To investigate the role of IFNG-AS1 in the molecular mechanisms of pituitary adenoma.
  • To identify potential molecular targets for PA treatment.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) and RNA fluorescence in situ hybridization (FISH) were used to assess IFNG-AS1 expression.
  • shRNA-mediated knockdown and overexpression techniques were employed in HP75 cells to study the functional impact of IFNG-AS1 and ESRP2.
  • Interaction between IFNG-AS1 and Epithelial splicing regulatory protein 2 (ESRP2) was investigated.

Main Results:

  • IFNG-AS1 expression was significantly elevated in PA tissues compared to non-tumor tissues.
  • Knockdown of IFNG-AS1 inhibited tumor progression, while its overexpression promoted it.
  • ESRP2 was identified as a target of IFNG-AS1, with its modulation affecting tumor progression in response to IFNG-AS1 manipulation.

Conclusions:

  • IFNG-AS1 functions as an oncogene in pituitary adenoma.
  • The oncogenic role of IFNG-AS1 in PA is mediated through its interaction with ESRP2.
  • Targeting the IFNG-AS1/ESRP2 pathway may offer a novel therapeutic approach for pituitary adenoma.
Keywords:
Epithelial splicing regulatory protein 2 (ESRP2)Long noncoding RNA IFNG-AS1 (IFNG-AS1)OncogenePituitary adenoma (PA)

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