In vitro thermotherapy-based methods for plant virus eradication

Area of Science:

• Plant Pathology
• Horticulture
• Biotechnology

Background:

• Virus-free plants are essential for disease control, germplasm exchange, and importing new cultivars.
• In vitro techniques are the most effective methods for eradicating plant viruses.
• Thermotherapy-based in vitro methods have shown success in eliminating various viruses from important crops.

Purpose of the Study:

• To review recent advancements in in vitro thermotherapy-based virus eradication methods.
• To discuss the mechanisms behind the efficacy of thermotherapy in virus eradication.
• To propose future research directions in this field.

Main Methods:

• Review of recent scientific literature on in vitro thermotherapy for virus eradication.
• Analysis of combined techniques such as thermotherapy with shoot tip culture, chemotherapy, micrografting, and cryotherapy.
• Discussion of the underlying mechanisms of virus elimination through thermotherapy.

Main Results:

• In vitro thermotherapy, often combined with other techniques, is highly effective for eradicating viruses from economically important crops.
• Significant progress has been made in refining these methods since the 21st century.
• Understanding the mechanisms enhances the efficiency and application of these techniques.

Conclusions:

• In vitro thermotherapy-based methods are a cornerstone for producing virus-free plants.
• Continued research into mechanisms and novel combinations will further improve virus eradication strategies.
• These techniques are vital for global plant health, trade, and agricultural sustainability.