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MicroRNA-129-5p inhibits human glioma cell proliferation and induces cell cycle arrest by directly targeting DNMT3A

Xuhui Gu1, Hui Gong1, Lili Shen1

  • 1Department of Neurosurgery, Haimen People's Hospital Haimen 226100, Jiangsu Province, China.

American Journal of Translational Research
|October 17, 2018
PubMed
Abstract

Insights

MicroRNA-129-5p acts as a tumor suppressor in glioma by targeting DNMT3A. Lower miR-129-5p levels correlate with higher glioma grade, suggesting its therapeutic potential.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Glioma is a prevalent and aggressive brain tumor with poor patient outcomes.
  • MicroRNAs (miRNAs) are implicated in cancer development, offering potential therapeutic avenues for glioma.
  • The precise roles and mechanisms of miRNAs in glioma remain incompletely understood.

Purpose of the Study:

  • To investigate the function of miR-129-5p in glioma.
  • To identify the direct molecular targets of miR-129-5p in glioma cells.
  • To explore the therapeutic potential of miR-129-5p in glioma treatment.

Main Methods:

  • Analysis of miR-129-5p and DNMT3A expression in glioma databases and patient samples.
  • Quantitative reverse transcription-PCR and Western blotting to assess expression levels.
  • Cellular assays (CCK-8, EDU, flow cytometry) to evaluate proliferation and cell cycle effects.
  • Luciferase reporter assays and rescue experiments to confirm direct targeting of DNMT3A by miR-129-5p.

Main Results:

  • miR-129-5p expression was significantly reduced in glioma tissues and inversely correlated with tumor grade.
  • Overexpression of miR-129-5p inhibited glioma cell proliferation and induced G1 cell cycle arrest.
  • DNA (cytosine-5)-methyltransferase 3A (DNMT3A) was validated as a direct target of miR-129-5p.
  • miR-129-5p exerts its tumor-suppressive effects by regulating DNMT3A in glioma cells.

Conclusions:

  • miR-129-5p functions as a tumor suppressor in glioma by directly inhibiting DNMT3A.
  • This miR-129-5p/DNMT3A axis represents a potential novel therapeutic target for glioma.
  • The findings suggest a new treatment strategy for glioma and other cancers driven by DNMT3A.
Keywords:
DNMT3AgliomamiR-129-5pproliferation

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