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Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
Published on: June 13, 2016
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Differentially circulating exosomal microRNAs expression profiling in oral lichen planus.
Qiao Peng1, Jing Zhang1,2, Gang Zhou1,2
1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University Wuhan, P. R. China.
American Journal of Translational Research
|October 17, 2018
Summary
Circulating exosomal miR-34a-5p may serve as a biomarker for oral lichen planus (OLP) severity. This study identified specific exosomal microRNAs (miRNAs) linked to OLP, with miR-34a-5p showing a positive correlation with disease severity.
Area of Science:
- Immunology
- Genetics
- Biochemistry
Background:
- Oral lichen planus (OLP) is a prevalent chronic inflammatory autoimmune condition with an unknown cause.
- Exosomes contain microRNAs (miRNAs) that can influence immune and inflammatory responses.
Purpose of the Study:
- To investigate the expression profiles of circulating exosomal miRNAs in OLP patients.
- To identify potential exosomal miRNA biomarkers for OLP severity.
Main Methods:
- Plasma exosomes were isolated from OLP patients and healthy controls.
- miRNA expression was analyzed using miScript® miRNA PCR Array and RT-PCR.
- The RAE scoring system assessed OLP severity.
- Bioinformatics analysis predicted target genes and pathways.
Main Results:
- Circulating exosomal miR-34a-5p and miR-130b-3p were upregulated in OLP patients.
- Exosomal miR-301b-3p was downregulated in OLP patients.
- Exosomal miR-34a-5p levels positively correlated with OLP severity.
- miR-34a-5p targets genes involved in gene regulation, cell communication, and signaling pathways like PI3K/Akt.
Conclusions:
- Circulating exosomal miR-34a-5p is a potential biomarker for assessing OLP severity.
- Exosomal miRNAs play a role in the pathogenesis of OLP.
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