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Updated: Feb 3, 2026

In vivo Calcium Imaging in Mouse Inferior Olive
Published on: June 10, 2021
Development and Characterization of a New Cell Line from Olive Flounder Paralichthys olivaceus
Ju-Won Kim1, Bang Geun Oh1, Julan Kim1
1Biotechnology Research Division, National Institute of Fisheries Science, Busan 46083, Korea.
Abstract:
A new embryonic cell line (OFEC-17FEN) derived from olive flounder Paralichthys olivaceus was developed. OFEC-17FEN cells were subcultured for <30 passages over ~200 days. OFEC-17FEN cells had a doubling time of 114.34 h and modal diploid chromosome number was 48. The pluripotency genes POU5f1 and NANOG were expressed in OFEC-17FEN cells. However, the lack of several pluripotency-related genes expression indicates that OFEC-17FEN cells are not stem cells. OFEC-17FEN cells transfected with plasmid pEGFP-c1 exhibited a strong green fluorescent signal at 48 h after transfection. Accordingly, OFEC-17FEN cells may be useful for both basic research and biotechnological application.
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