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Related Experiment Video

Updated: Feb 3, 2026

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Cell Death Analysis in Retinal Cultures.

Sarah L Roche1, Ana M Ruiz-Lopez1, Thomas G Cotter2

  • 1Tumour Biology Laboratory, School of Biochemistry and Cell Biology, Bioscience Research Institute, University College Cork, Cork, Ireland.

Methods in Molecular Biology (Clifton, N.J.)
|October 17, 2018
PubMed
Summary

This study details two Terminal dUTP nick end-labeling (TUNEL) methods for quantifying retinal cell death. These techniques aid in neurodegeneration and neuroprotection research by identifying dying cells in retinal explants and cell lines.

Keywords:
661WFlow cytometryFluorescence microscopyPhotoreceptor-like cell lineRetinal explantTUNEL

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Ophthalmology

Background:

  • Evaluating cell death is crucial for understanding retinal neurodegeneration and neuroprotection.
  • Cell death assays are vital for identifying and quantifying dying cells within a population.

Purpose of the Study:

  • To describe two distinct Terminal dUTP nick end-labeling (TUNEL) techniques for assessing cell death in retinal tissues.
  • To provide methodologies for researchers investigating retinal cell death.

Main Methods:

  • Utilized Terminal dUTP nick end-labeling (TUNEL) assay for detecting fragmented DNA, indicative of cell death.
  • Applied fluorescence microscopy to analyze cell death in cultured retinal explants.
  • Employed flow cytometry to measure cell death in a retinal cell line.

Main Results:

  • Successfully demonstrated TUNEL assay application in two distinct retinal models.
  • Provided validated protocols for quantifying retinal cell death using TUNEL.

Conclusions:

  • The described TUNEL techniques offer reliable methods for assessing retinal cell death.
  • These methods are applicable to both cultured retinal explants and cell lines, supporting neurodegeneration research.