Related Experiment Video

Updated: Feb 3, 2026

Stimulation of Vascular Endothelial Cells Using Neutrophil Extracellular Traps in the Presence of Low-Density Lipoprotein
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Stimulation of Vascular Endothelial Cells Using Neutrophil Extracellular Traps in the Presence of Low-Density Lipoprotein

Published on: August 12, 2025

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MicroRNA-328 ameliorates oxidized low-density lipoprotein-induced endothelial cells injury through targeting HMGB1 in

Chun-Yang Wu1, Zhao-Feng Zhou1, Bin Wang1

  • 1Department of Cardiology, Yancheng Hospital Affiliated to Southeast University School of Medicine, Yancheng, China.

Journal of Cellular Biochemistry
|October 17, 2018
PubMed

Insights

MicroRNA-328 protects against atherosclerosis by reducing inflammation and oxidative stress in endothelial cells. It targets high-mobility group box-1 (HMGB1), mitigating damage caused by oxidized low-density lipoprotein (ox-LDL).

Area of Science:

  • Cardiovascular Biology
  • Molecular Medicine
  • Cellular Pathology

Background:

  • Atherosclerosis is a chronic inflammatory disease impacting blood vessels.
  • MicroRNAs (miRNAs) are key regulators in various diseases, including atherosclerosis.
  • The specific role of miR-328 in atherosclerosis has been underexplored.

Purpose of the Study:

  • To investigate the role of miR-328 in endothelial cell injury during atherosclerosis.
  • To determine how oxidized low-density lipoprotein (ox-LDL) affects miR-328 expression and endothelial cell function.
  • To identify downstream targets of miR-328 involved in atherosclerosis.

Main Methods:

  • Oxidized low-density lipoprotein (ox-LDL) was used to induce injury in human umbilical vein endothelial cells (HUVECs).
  • Cell viability, apoptosis, inflammation, and oxidative stress were assessed.
  • miR-328 mimics and inhibitors were used to modulate miR-328 levels.
  • Bioinformatics analysis and experimental validation were employed to identify miR-328 targets.

Main Results:

  • ox-LDL induced HUVEC apoptosis, reduced cell viability, and promoted inflammation and oxidative stress.
  • ox-LDL treatment decreased miR-328 expression in HUVECs.
  • Overexpression of miR-328 repressed ox-LDL-induced apoptosis and inflammation, while enhancing cell survival.
  • Inhibition of miR-328 exacerbated ox-LDL-induced endothelial cell injury.
  • High-mobility group box-1 (HMGB1) was identified as a direct downstream target of miR-328.

Conclusions:

  • miR-328 plays a protective role against ox-LDL-induced endothelial cell injury in atherosclerosis.
  • miR-328 ameliorates endothelial cell damage by targeting HMGB1.
  • These findings highlight miR-328 as a potential therapeutic target for atherosclerosis.
Keywords:
atherosclerosishigh-mobility group box-1microRNA-328oxidized low-density lipoprotein

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