Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Endocrine Signaling01:45

Endocrine Signaling

68.1K
Endocrine cells produce hormones to communicate with remote target cells found in other organs. The hormone reaches these distant areas using the circulatory system. This exposes the whole organism to the hormone but only those cells expressing hormone receptors or target cells are affected. Thus, endocrine signaling induces slow responses from its target cells but these effects also last longer.
68.1K
Bacterial Signaling01:30

Bacterial Signaling

40.7K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
40.7K
Yeast Signaling01:28

Yeast Signaling

17.3K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
17.3K
What is Cell Signaling?02:03

What is Cell Signaling?

130.4K
Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate to respond to the environment.
130.4K
Paracrine Signaling01:21

Paracrine Signaling

59.6K
Paracrine signaling allows cells to communicate with their immediate neighbors via secretion of signaling molecules. Such a signal can only trigger a response in nearby target cells because the signal molecules degrade quickly or are inactivated if not taken up. Prominent examples of paracrine signaling include nitric oxide signaling in blood vessels, synaptic signaling of neurons, the blood clotting system, tissue repair/wound healing, and local allergic skin reactions. Nitric oxide as a...
59.6K
Synaptic Signaling01:12

Synaptic Signaling

79.5K
Neurons communicate at synapses, or junctions, to excite or inhibit the activity of other neurons or target cells, such as muscles. Synapses may be chemical or electrical.
79.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A longitudinal, multi-omic atlas reveals the emergence of a spatially organized immunosuppressive ecosystem in resistant melanoma.

Cell reports. Medicine·2026
Same author

Risk of Occult Contralateral Neck Metastasis in Early-Stage HPV-Related Lateralized Cancer of the Base of the Tongue.

Head & neck·2025
Same author

Method for generation and ex vivo expansion of genetically edited mouse Tregs.

Journal of leukocyte biology·2025
Same author

Outcomes of HPV+ Oropharyngeal Carcinoma of Unknown Primary Following Transoral Robotic Surgery.

The Laryngoscope·2025
Same author

Human Extravillous Trophoblasts Require SRC-2 for Sustained Viability, Migration, and Invasion.

Cells·2025
Same author

Long-Term Follow-Up of E3311, an ECOG-ACRIN Cancer Research Group Phase II Trial of Transoral Surgery and Risk-Based Adjuvant Treatment in Human Papillomavirus-Initiated Oropharynx Cancer.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2025

Related Experiment Video

Updated: Feb 3, 2026

Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization
14:55

Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization

Published on: September 1, 2018

11.1K

Retinoid signaling controlled by SRC-2 in decidualization revealed by transcriptomics

Maria M Szwarc1, Lan Hai1, William E Gibbons2

  • 1Department of Molecular & Cellular Biology, Baylor College of Medicine, Houston, Texas, USA.

Reproduction (Cambridge, England)
|October 17, 2018
PubMed
Summary

Steroid receptor coactivator-2 (SRC-2) is crucial for progesterone receptor (PGR)-driven decidualization in human endometrial stromal cells (hESCs). SRC-2 regulates genes vital for decidualization, including those involved in retinoid signaling essential for successful pregnancy.

More Related Videos

Isolation of Lung Retinoid-Containing Cells by Cell Sorting
07:47

Isolation of Lung Retinoid-Containing Cells by Cell Sorting

Published on: April 11, 2025

707
Generation of a Mouse Artificial Decidualization Model with Ovariectomy for Endometrial Decidualization Research
06:38

Generation of a Mouse Artificial Decidualization Model with Ovariectomy for Endometrial Decidualization Research

Published on: July 27, 2022

6.3K

Related Experiment Videos

Last Updated: Feb 3, 2026

Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization
14:55

Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization

Published on: September 1, 2018

11.1K
Isolation of Lung Retinoid-Containing Cells by Cell Sorting
07:47

Isolation of Lung Retinoid-Containing Cells by Cell Sorting

Published on: April 11, 2025

707
Generation of a Mouse Artificial Decidualization Model with Ovariectomy for Endometrial Decidualization Research
06:38

Generation of a Mouse Artificial Decidualization Model with Ovariectomy for Endometrial Decidualization Research

Published on: July 27, 2022

6.3K

Area of Science:

  • Reproductive biology
  • Molecular endocrinology
  • Genetics

Background:

  • Successful pregnancy depends on embryo implantation and uterine receptivity.
  • Progesterone receptor (PGR)-mediated decidualization of endometrial stromal cells (ESCs) is critical for supporting embryo development and placentation.
  • Steroid receptor coactivator-2 (SRC-2) has been identified as a key regulator of PGR activity in endometrial decidualization.

Purpose of the Study:

  • To comprehensively identify the genes regulated by SRC-2 in decidualizing human ESCs (hESCs).
  • To elucidate the role of SRC-2 in the transcriptional network governing endometrial decidualization.
  • To investigate the functional pathways influenced by SRC-2 during progesterone-driven decidualization.

Main Methods:

  • RNA sequencing and chromatin immunoprecipitation sequencing (ChIP-seq) were employed to profile the transcriptome and identify SRC-2 binding sites in decidualizing hESCs.
  • Bioinformatic analysis, including gene ontology (GO) analysis, was used to interpret the functional significance of SRC-2-regulated genes.
  • Specific gene expression and functional assays were performed to validate key findings, particularly concerning retinoid signaling.

Main Results:

  • The study identified a significant number of SRC-2-regulated genes in hESCs, with the majority directly involved in the decidualization process.
  • Over 50% of SRC-2-dependent genes were found to be co-regulated by the PGR, highlighting a significant overlap in their regulatory networks.
  • SRC-2 was found to be essential for the induction of retinoid signaling pathways, specifically regulating the expression of the retinol transporter STRA6, which is critical for hESC decidualization.

Conclusions:

  • SRC-2 plays a critical role in mediating PGR-driven transcriptional reprogramming during human endometrial decidualization.
  • SRC-2 regulates a substantial subset of genes essential for decidualization, impacting various cellular processes.
  • The findings underscore the importance of SRC-2 in maintaining retinoid signaling, a pathway vital for successful progesterone-mediated decidualization and potentially early pregnancy.