Related Experiment Video
Updated: Feb 3, 2026

Measuring the Shape and Size of Activated Sludge Particles Immobilized in Agar with an Open Source Software Pipeline
Published on: January 30, 2019
Overcoming floc formation limitations in high-rate activated sludge systems
Tim Van Winckel1, Xiaocen Liu2, Siegfried E Vlaeminck3
1Center of Microbial Ecology and Technology (CMET), Faculty of Bioscience Engineering, Ghent University, Gent, Belgium; District of Columbia Water and Sewer Authority, Blue Plains Advanced Wastewater Treatment Plant, 5000 Overlook Ave, SW Washington, DC 20032, USA; Department of Civil, Environmental and Architectural Engineering, The University of Kansas, KS, USA.
High-rate activated sludge (HRAS) systems struggle with organic capture due to poor floc formation. This study identified and solved limitations by adjusting sludge properties, improving solid separation for energy-positive wastewater treatment.
Area of Science:
- Environmental Engineering
- Wastewater Treatment Technologies
- Biotechnology
Background:
- High-rate activated sludge (HRAS) systems aim for energy-positive sewage treatment by maximizing organic capture.
- Effective solid-liquid separation in clarifiers is crucial for HRAS performance.
- Flocculation limitations often hinder organic capture in HRAS, impacting overall efficiency.
Purpose of the Study:
- To identify and overcome floc formation limitations in full-scale HRAS reactors.
- To analyze the impact of shear, sludge concentration, and chemical additives on flocculation.
- To develop an analysis toolkit for addressing solid separation challenges in HRAS.
Main Methods:
- Conducted orthokinetic flocculation tests under varied conditions (shear, sludge concentration, coagulant/flocculant).
- Utilized traditional and novel settling parameters and extracellular polymeric substances (EPS) measurements.
- Employed bio-augmentation with sludge from a long solids retention time (SRT) reactor.
Main Results:
- HRAS limitations were primarily due to insufficient collision efficiency, linked to short SRT and high colloid loading.
- Impaired flocculation, driven by EPS composition (low protein/polysaccharide ratio), limited collision efficiency.
- Bio-augmentation resolved collision efficiency issues but introduced floc strength limitations related to EPS amount and morphology.
Conclusions:
- Flocculation, not coagulation, was the main limitation in the studied HRAS system.
- EPS composition is critical for collision efficiency, while EPS amount influences floc strength.
- The proposed analysis toolkit aids in optimizing HRAS solid separation and organic recovery.
More Related Videos
06:11Author Spotlight: Advancing Anaerobic Microbiota Research Using a Novel Respirometry Protocol
Published on: April 26, 2024
15:19Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
Published on: October 15, 2015
Related Concept Videos
Functional Brain Systems: Reticular Formation
Within the reticular formation, there are several distinct nuclei that can be classified into three broad categories. The Raphe nuclei are located along the midline of the brainstem. They are primarily known for their role in synthesizing and releasing serotonin, a neurotransmitter involved in regulating mood, appetite, sleep, and circadian rhythms. The...
Rate-Determining Steps
In a multistep reaction mechanism, one of the elementary steps progresses significantly slower than the others. This slowest step is called the rate-limiting step (or rate-determining step). A reaction cannot proceed faster than its slowest step, and hence, the rate-determining step limits the overall reaction rate.
The concept of rate-determining step can be understood from the analogy of a 4-lane freeway with a short-stretch of traffic-bottleneck caused due to...
Limiting Reactant
Temperature Dependence on Reaction Rate
Atoms, molecules, or ions must collide before they can react with each other. Atoms must be close together to form chemical bonds. This premise is the basis for a theory that explains many observations regarding chemical kinetics, including factors affecting reaction rates.
The collision theory is based on the postulates that (i) the reaction rate is proportional to the rate of reactant collisions, (ii) the reacting species collide in an orientation allowing contact between...
The Number e as a Limit
Reaction Rate
The mathematical representation of the change in the concentration of reactants and products, over time, is the rate...