Photoaffinity labeling of a novel benzodiazepine binding protein in rat brain

Photoaffinity labeling experiments were conducted with [3H]clonazepam to investigate the possible existence of a benzodiazepine binding site that is distinct from the central- and peripheral-type benzodiazepine binding sites. These studies demonstrate the presence of a novel benzodiazepine binding protein in rat brain with a relative mobility of 65,000 daltons that binds benzodiazepines stereoselectivity in the high nanomolar-low micromolar concentration range.