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Yeast: bridging the gap between phenotypic and biochemical assays for high-throughput screening
1a Department of Biosciences and Centre for Global Infectious Disease , Durham University , Durham , UK.
Expert Opinion on Drug Discovery
|October 18, 2018
Summary
Yeast-based high throughput screening (HTS) offers a eukaryotic cellular context for drug discovery, overcoming limitations of traditional assays. These platforms show potential to bridge the gap between phenotypic and biochemical screening methods.
Area of Science:
- Biochemistry
- Molecular Biology
- Drug Discovery
Background:
- Traditional in vitro biochemical and phenotypic assay platforms have inherent limitations for high throughput screening (HTS) in drug discovery.
- Genetically tractable model yeast provides a eukaryotic cellular context for target-based HTS, enabling identification of on-target compounds.
Purpose of the Study:
- To discuss the utilization of yeast-based assays in HTS over the past 20 years.
- To review platforms, counter/secondary screening, and detection system advancements for miniaturization and ultra-HTS.
Main Methods:
- Review of yeast-based assay platforms used in HTS.
- Discussion of counter and secondary screening strategies.
- Analysis of recent developments in detection systems for miniaturization.
Main Results:
- Yeast assays overcome limitations of in vitro methods by providing a cellular context.
- Counter and secondary screening are essential for confirming on-target activity.
- Detection system advancements enable miniaturization and ultra-high throughput screening.
Conclusions:
- Yeast-based HTS platforms demonstrate utility in industrial settings.
- These platforms have the potential to bridge the gap between phenotypic and biochemical assays.
- Optimizing yeast-based HTS offers significant promise for drug discovery.
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