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Updated: Feb 3, 2026
10:04
Establishment of Proliferative Tetraploid Cells from Nontransformed Human Fibroblasts
Published on: January 8, 2017
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An interspecies barrier to tetraploid complementation and chimera formation
Tomoyuki Yamaguchi1, Hideyuki Sato2, Toshihiro Kobayashi2,3
1Division of Stem Cell Therapy, Institute of Medical Science, University of Tokyo, Tokyo, 108-8639, Japan. tomoyama@ims.u-tokyo.ac.jp.
Scientific Reports
|October 18, 2018
Summary
Mouse and rat stem cells formed fewer chimeras across species than within species. High donor cell contribution led to developmental issues or death, indicating species-specific molecular interactions are crucial for development.
Area of Science:
- Developmental Biology
- Stem Cell Biology
- Comparative Embryology
Background:
- Understanding conceptus development in xenogeneic environments is crucial for regenerative medicine and interspecies research.
- Previous studies have explored intraspecies chimerism, but xenogeneic development presents unique challenges.
Purpose of the Study:
- To investigate the feasibility and outcomes of interspecies chimera formation between mouse and rat.
- To assess tetraploid complementation efficiency in a mouse-rat xenogeneic model.
- To identify factors influencing donor cell contribution and developmental success in interspecies hybrids.
Main Methods:
- Generation of interspecies chimeras by aggregating mouse and rat pluripotent stem cells.
- Utilized tetraploid complementation assay with mouse and rat embryos.
- Quantified donor cell contribution across various tissues and developmental stages.
Main Results:
- Donor stem cell contribution was significantly lower in interspecies chimeras compared to intraspecies controls.
- High levels of donor chimerism were correlated with developmental anomalies and embryonic lethality.
- Significant organ-to-organ variation in donor chimerism was observed, suggesting species-specific molecular incompatibilities.
- Interspecies tetraploid complementation resulted in normal early development, but failed at placental formation, leading to embryonic death.
Conclusions:
- Mouse and rat stem cells can form interspecies chimeras, but with limited donor cell contribution and developmental potential.
- Species-specific molecular interactions are critical for successful organogenesis and placental development in xenogeneic environments.
- These findings highlight significant barriers to interspecies developmental compatibility.
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