Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chromosome analysis by two-dimensional fingerprinting.

S K Poddar, J Maniloff

    Gene
    |January 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Guidelines to the demarcation of virus species.

    Archives of virology·2017
    Same author

    Modification of modified young's operation in the management of primary atrophic rhinitis.

    Indian journal of otolaryngology and head and neck surgery : official publication of the Association of Otolaryngologists of India·2012
    Same author

    Differential detection of B. pertussis from B. parapertussis using a polymerase chain reaction (PCR) in presence of SYBR green1 and amplicon melting analysis.

    Molecular and cellular probes·2004
    Same author

    Rapid detection of Bordetella pertussis by real-time PCR using SYBR green I and a LightCycler instrument.

    Journal of clinical laboratory analysis·2004
    Same author

    Detection and discrimination of B pertussis and B holmesii by real-time PCR targeting IS481 using a beacon probe and probe-target melting analysis.

    Molecular and cellular probes·2003
    Same author

    Taxonomic proposals on the Web: new ICTV consultative procedures.

    Archives of virology·2003

    A new two-dimensional DNA fingerprinting method analyzes large genomes using restriction enzyme digestion and gel electrophoresis. This technique accurately determines genome size and cleavage sites, validated in bacterial and mycoplasma strains.

    Area of Science:

    • Molecular Biology
    • Genomics
    • Biotechnology

    Background:

    • Analyzing large DNA molecules requires precise methods for genome characterization.
    • Restriction endonuclease digestion and gel electrophoresis are foundational techniques in molecular biology.

    Purpose of the Study:

    • To develop and validate a novel two-dimensional DNA fingerprinting technique for analyzing large, cell genome-size DNA.
    • To establish equations for calculating genome size and restriction enzyme cleavage sites from fragment length distributions.

    Main Methods:

    • Two-dimensional DNA fingerprinting involving restriction endonuclease cleavage.
    • Separation of DNA fragments using agarose gel electrophoresis.
    • Derivation of equations to quantify genome size and cleavage sites from fragment length data.

    Related Experiment Videos

    Main Results:

    • The developed two-dimensional fingerprinting technique successfully analyzed large DNA fragments.
    • Genome sizes for Escherichia coli strain JM101 and Acholeplasma laidlawii strains were accurately determined.
    • Measured genome sizes agreed with previously published values, validating the method.

    Conclusions:

    • The novel two-dimensional fingerprinting technique provides a reliable method for analyzing prokaryotic and eukaryotic genome structures.
    • This approach offers potential for diverse applications in genome organization studies.
    • The method's accuracy is confirmed by its agreement with established data for bacterial and mycoplasma genomes.