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CD133 expression in circulating hematopoietic progenitor cells.

Thomas R Cimato1, Alexis Conway2, Julianne Nichols1

  • 1Department of Medicine/Division of Cardiology, University at Buffalo, Jacobs School of Medicine and Biomedical Sciences, Buffalo, New York.

Cytometry. Part B, Clinical Cytometry
|October 18, 2018
PubMed
Summary
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Most circulating CD34+ cells lack CD133. CD34+ CD133+ cells represent early hematopoietic stem cells and progenitors, distinct from lineage-specified progenitors in healthy adults.

Keywords:
Flow cytometryHematopoietic stem cells

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Area of Science:

  • Hematology
  • Immunology
  • Cell Biology

Background:

  • Circulating hematopoietic progenitors (HPCs) are crucial in inflammatory diseases, cancer immunity, and hematopoietic disorders.
  • Flow cytometry, using CD34 and other markers, identifies HPCs for lineage determination.
  • CD133 (prominin-1) is another HPC marker with overlapping, but not identical, expression to CD34.

Purpose of the Study:

  • To investigate the distribution of CD133 expression within circulating CD34+ HPC subtypes.
  • To characterize the composition of CD34+ CD133+ and CD34+ CD133- cell populations.

Main Methods:

  • Utilized multicolor flow cytometry to quantify HPC populations.
  • Analyzed venous blood from healthy human subjects.
  • Differentiated between CD34+ CD133+ and CD34+ CD133- fractions.

Main Results:

  • The majority of circulating CD34+ cells were found to be CD133-.
  • CD34+ CD133+ cells exhibited low CD38 expression.
  • CD34+ CD133+ cells contained stem cell and multipotent progenitor populations, largely lacking CD38+ lineage-specified progenitors.

Conclusions:

  • Clarified the composition of circulating CD133+ CD34+ cell types in adult humans.
  • Demonstrated that CD34+ CD133+ cells represent early progenitor populations.
  • Highlighted the distinct nature of CD34+ CD133+ cells compared to lineage-specified progenitors.