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Development of a fluvoxamine detection system using a Quenchbody, a novel fluorescent biosensor
Ako Sasao1, Michiyo Takaki1, Hee-Jin Jeong2
1Department of Forensic Medicine, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan.
Drug Testing and Analysis
|October 18, 2018
Summary
A new fluoroimmunosensor, Quenchbody (Q-body), rapidly detects the antidepressant fluvoxamine (FLV). Optimized N-terminus labeled Q-bodies show high sensitivity in serum after protein removal, aiding drug misuse monitoring.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Pharmacology
Background:
- Psychotropic drug misuse is a growing global public health issue.
- Accurate and rapid detection methods are crucial for therapeutic drug monitoring and forensic analysis.
- Novel fluoroimmunosensors offer potential for sensitive and immediate detection of target analytes.
Purpose of the Study:
- To develop and optimize Quenchbody (Q-body) fluoroimmunosensors for the detection of fluvoxamine (FLV).
- To evaluate the performance of Q-bodies in different configurations and matrices.
- To assess the feasibility of using Q-bodies for detecting FLV in biological samples like serum.
Main Methods:
- Synthesis of five Q-bodies with varying fluorophore N- or C-terminal labeling and linker lengths.
- Measurement of fluorescence intensity (FI) upon interaction with FLV.
- Determination of optimal labeling strategies and conditions for maximum FI ratio.
- Evaluation of Q-body performance in phosphate-buffered saline and serum matrices, including assessment of deproteinization effects.
Main Results:
- N-terminus labeled Q-bodies exhibited a ~1.5-fold increase in normalized fluorescence intensity (FI ratio) upon FLV addition.
- C-terminus labeled Q-bodies showed no significant increase in FI.
- Rhodamine 6G labeled Q-body demonstrated the best FI ratio.
- Effective detection of FLV in serum filtrate was achieved after deproteinization, showing dose-dependency.
Conclusions:
- Optimized N-terminus labeled Q-bodies are effective for rapid FLV detection.
- Deproteinization is necessary for sensitive FLV detection in serum using Q-bodies.
- Q-body technology holds promise for developing innovative immunoassay applications for drug monitoring.
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