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An in vitro assessment of bacterial transfer by products used in debridement

Mark G Rippon1, Alan A Rogers2, Laura Sellars3

  • 1Visiting Clinical Research Fellow; Huddersfield University, Queensgate, Huddersfield.

Journal of Wound Care
|October 18, 2018
PubMed
Abstract

Insights

Mechanical debridement tools spread more Pseudomonas aeruginosa biofilm than hydroresponsive wound dressing with polyhexamethylene biguanide (HRWD+PHMB). HRWD+PHMB dressing showed minimal microorganism transfer, suggesting it is safer for wound biofilm management.

Area of Science:

  • Wound Care
  • Microbiology
  • Biomaterials

Background:

  • Pseudomonas aeruginosa biofilms pose a significant challenge in wound healing.
  • Effective debridement is crucial for managing biofilm infections.
  • Understanding microbial transfer during wound treatment is vital for preventing spread.

Purpose of the Study:

  • To investigate the in vitro transfer of viable Pseudomonas aeruginosa biofilm microorganisms.
  • To compare microbial transfer by mechanical debridement tools versus a hydroresponsive wound dressing (HRWD+PHMB).

Main Methods:

  • In vitro study design.
  • Simulated clinical practice for debridement tool and HRWD+PHMB application.
  • Quantification of viable biofilm microorganisms transferred by each treatment method.

Main Results:

  • Mechanical debridement tools showed significant transfer of Pseudomonas aeruginosa.
  • HRWD+PHMB demonstrated minimal microorganism transfer.
  • Fewer bacteria were recovered from explants treated with HRWD+PHMB compared to debridement tools.

Conclusions:

  • HRWD+PHMB effectively binds, retains, and kills Pseudomonas aeruginosa, reducing microbial transfer.
  • Mechanical debridement tools likely contribute to bacterial spread in clinical settings.
  • HRWD+PHMB offers a potentially safer alternative for managing biofilm in wounds.
Keywords:
Pseudomonas aeruginosahydroresponsive wound dressingslaboratory investigationsmechanical antimicrobial action

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