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Anticancer and Anti-Inflammatory Activities of Some New Pyrazolo[3,4-b]pyrazines
Hussein El-Kashef1, Talaat El-Emary2, Pierre Verhaeghe3
1Chemistry Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt. elkashef@aun.edu.eg.
Abstract:
New derivatives of pyrazolo[3,4-b]pyrazines and related heterocycles were synthesized using 5-amino-3-methyl-4-nitroso-1-phenyl-pyrazole (1) as a starting material. The 5-acetyl derivative 15 was shown to be a useful key intermediate for the synthesis of several derivatives of pyrazolopyrazines. Some of the prepared compounds were evaluated for their anti-inflammatory and anti-breast cancer MCF-7 cell line activities. SAR study showed that compounds 15 and 29 exhibited remarkable anti-inflammatory activity, where 15 showed the same activity as that of the reference drug indomethacin. On the other hand, compounds 25i, 25j showed very significant inhibitory activity (p < 0.001) against MCF-7 breast cancer cell line.
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