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Related Concept Videos

Horizontal Gene Transfer01:27

Horizontal Gene Transfer

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Horizontal gene transfer (HGT) is a process where genetic material moves between organisms within the same generation, unlike vertical gene transfer, which occurs from parent to offspring. HGT plays a crucial role in microbial evolution, adaptation, and survival, particularly in shared environments like the human gut.Mobile genetic elements such as plasmids, prophages, integrons, insertion sequences, and transposons facilitate this process. HGT occurs through three primary mechanisms:...
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Gene Therapy00:59

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Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
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Continuing care describes the variety of health, personal, and social services provided over a prolonged period. The need for continuing care is increasing because people are living longer. Many people do not have families or others to care for them. Continuing care is mainly for patients who are disabled, functionally dependent, or suffering from a terminal disease. It is available within institutional settings or in homes. Examples include nursing centers or facilities, assisted living,...
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Continuity of a Function01:23

Continuity of a Function

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A function is continuous at a point a if three conditions are met: the function is defined at a, the limit of the function as x approaches a exists, and this limit equals the function’s value. Mathematically, this is written asThis definition ensures the graph of the function does not exhibit any breaks, holes, or jumps at that point. Discontinuities occur when any of these conditions fail. A removable discontinuity exists when the two-sided limit exists but the function is either...
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Continuity Equation01:28

Continuity Equation

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The continuity equation asserts that the mass flow rate must remain constant for a steady flow of an incompressible fluid within a confined system. This principle applies to systems where fluid passes through varying cross-sectional areas, such as nozzles, syringes, and pipes.
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Continuity Equation01:20

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The total amount of current flowing per unit cross-sectional area is called the current density. Hence, the current passing through a cross-sectional area can be written as the surface integral of the current density.
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Related Experiment Video

Updated: Feb 3, 2026

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays
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Continuous Vector-free Gene Transfer with a Novel Microfluidic Chip and Nanoneedle Array.

Dong Huang1, Deyao Zhao1, Jinhui Li1

  • 1Institute of Molecular Medicine; Institute of Microelectronics, National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Peking University, Beijing 100871, China.

Current Drug Delivery
|October 19, 2018
PubMed
Summary

This study introduces a novel microfluidic chip for efficient gene delivery. The carrier-free platform achieves high transfection efficiency and cell viability for bioengineering and therapeutic applications.

Keywords:
Drug deliverychaotic microfluidicsgene therapymicrofluidic chipnanoneedle arraystaggered herringbone channel.

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A Microfluidic Chip for ICPMS Sample Introduction
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A Microfluidic Chip for ICPMS Sample Introduction
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Area of Science:

  • Bioengineering
  • Cellular Biology
  • Microfluidics

Background:

  • Efficient delivery of foreign molecules into cells is crucial for bioengineering and therapeutic advancements.
  • Current methods face limitations in throughput and efficiency for large-scale applications.

Purpose of the Study:

  • To develop a novel carrier-free gene delivery platform using a microfluidic chip.
  • To achieve high-throughput in vitro gene transfection with enhanced efficiency and cell viability.

Main Methods:

  • A microfluidic chip integrating staggered herringbone channels and a silicon nanoneedle array was designed.
  • Staggered herringbone grooves induced micro vortices, increasing cell-channel contact.
  • The nanoneedle array created transient cell membrane disruptions for cargo entry.

Main Results:

  • The microfluidic chip demonstrated high gene transfection efficiency exceeding 20%.
  • Excellent cell viability, above 95%, was maintained during the process.
  • Achieved flow-through treatment, outperforming conventional nanoneedle systems.

Conclusions:

  • The developed platform offers a continuous processing environment for large-scale biological molecule transfection.
  • This technology shows significant potential for both fundamental research and clinical applications.
  • The carrier-free approach enhances efficiency and viability in gene delivery systems.