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A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy

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mRNA as novel technology for passive immunotherapy.

Thomas Schlake¹, Andreas Thess², Moritz Thran²

¹CureVac AG, Paul-Ehrlich-Str. 15, 72076, Tübingen, Germany. thomas.schlake@curevac.com.

Cellular and Molecular Life Sciences : CMLS

|October 19, 2018

Summary

Messenger RNA (mRNA) offers a safe and effective method for passive immunotherapy by delivering therapeutic proteins. This technology provides advantages over traditional protein administration and DNA transfection for immunological interventions.

Keywords:

Adoptive cell transfer Antibody CAR T cell Immune globulin Passive immunization Tolerability mRNA design mRNA technology

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Area of Science:

Immunology
Molecular Biology
Biotechnology

Background:

Passive immunotherapy utilizes exogenous immunological effectors like antibodies or engineered lymphocytes.
Recombinant protein production is central to passive immunotherapy, with mRNA emerging as a key delivery vehicle.
In vitro transcribed (IVT) mRNA offers transient protein expression, distinct from permanent genetic modification.

Purpose of the Study:

To review the current status of IVT mRNA technology for therapeutic applications.
To explore the application of IVT mRNA in passive immunotherapy strategies.
To highlight the advantages of mRNA-based protein delivery for immunological interventions.

Main Methods:

Review of existing literature on passive immunotherapy and mRNA technology.
Analysis of the properties and advantages of IVT mRNA as a drug substance.
Evaluation of efficacy data from various models demonstrating mRNA's potential.

Main Results:

IVT mRNA serves as a transient carrier for protein translation, offering inherent safety advantages over DNA.
mRNA technology facilitates the production of difficult-to-manufacture proteins and can extend the pharmacokinetics of short-lived proteins.
Efficacy data support IVT mRNA as a potent and flexible platform for immunological interventions.

Conclusions:

IVT mRNA represents a significant advancement in drug delivery for passive immunotherapy.
The transient nature and safety profile of mRNA make it a promising alternative to traditional methods.
mRNA technology offers a versatile platform with broad applications in developing novel immunological treatments.