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Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
Published on: October 4, 2017
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Glycosylation control technologies for recombinant therapeutic proteins
Sanjeev K Gupta1, Pratyoosh Shukla2
1Adavanced Biotech Lab, Ipca Laboratories Ltd., Kandivali (West), Mumbai, India.
Applied Microbiology and Biotechnology
|October 19, 2018
Summary
Controlling protein glycosylation is crucial for biopharmaceutical efficacy and safety. This review explores glycoengineering and host selection to achieve desired glycoforms and minimize side effects.
Area of Science:
- Biopharmaceutical Manufacturing
- Glycobiology
- Protein Engineering
Background:
- Protein glycosylation significantly impacts biopharmaceutical efficacy, half-life, and antigenicity.
- Current expression systems often produce undesirable, non-human glycan structures, leading to adverse effects.
- Achieving uniform and desired glycan composition is a major challenge in recombinant glycoprotein production.
Purpose of the Study:
- To review current technologies for improving glycan composition in biotherapeutics.
- To highlight strategies for minimizing glycan heterogeneity in recombinant protein production.
- To guide the selection of expression hosts and glycoengineering approaches for enhanced biological activity.
Main Methods:
- Comprehensive review of glycoengineering strategies.
- Analysis of host selection for recombinant glycoprotein production.
- Evaluation of upstream process optimization for glycosylation control.
Main Results:
- Glycoengineering of mammalian, insect, and yeast systems can yield human-like glycan profiles.
- Host and clone selection are critical for achieving specific glycosylation patterns.
- Cell culture, biochemical, and physical process parameters influence glycoform manufacturing.
Conclusions:
- Optimizing expression hosts and glycoengineering are key to controlling protein glycosylation.
- Careful consideration of process parameters is essential for producing desired glycoforms.
- Targeted strategies can enhance biological activity and reduce side effects of biotherapeutics.
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