Improved membrane sequencing batch reactor: effect of carbon and nitrogen volumetric loading rate on dephosphatation

A lab-scale anaerobic-anoxic-aerobic membrane bioreactor (A₂NO-MBR) fed with synthetic wastewater was operated to investigate the impact of influent carbon and nitrogen volumetric loading rate (VLR) on dephosphatation, and the corresponding influent concentration was 100-300 mg L^-1 (COD), 24-50 mg L^-1 (NH₄⁺-N) and 4.8-6.0 mg L^-1 (TP), respectively. The results demonstrated that carbon VLR had a negligible effect on the COD removal with effluent below 50 mg L^-1, and high and stable removal capacity for phosphorus were also obtained, regardless of carbon VLR change. Whereas TN removal efficiency was positively correlated with carbon VLR reduction, and lower carbon VLR produced a negative effect on TN removal. In addition, since nitrate served as an electron acceptor for denitrifying phosphorus removal (DPR), a significant effect on nitrogen and phosphorus removal was observed with different nitrogen VLR. The TN and TP removal efficiency was 68.30 ± 1.36%, 70.70 ± 1.23%, 45.19 ± 1.72% and 41.63 ± 3.09%, 98.14 ± 0.53%, 53.34 ± 2.68% with influent nitrogen VLR of 0.024 ± 0.001, 0.034 ± 0.001 and 0.045 ± 0.001 kg-N/(m³ d), respectively. Moreover, bacterial community structure of sludge samples in Run I and V from anaerobic-anoxic-aerobic-SBR (named A₂OSBR_1 and A₂OSBR_2) and membrane bioreactor (named N-MBR_1 and N-MBR_2) revealed that Candidatus_Accumulibacter was the most dominant genus in A₂OSBR_1 (21.50%) and A₂OSBR_2 (18.98%). The relative lower carbon VLR favoured the enrichment of Saprospiraceae, which was related with DPR, with the proportion of 9.31% and 14.61% in A₂OSBR_1 and A₂OSBR_2. Besides, Nitrospira and Nitrosomonas with proportions of 11.14%, 5.38% in N-MBR_1 and 10.72%, 6.77% in N-MBR_2 were observed, which were likely responsible for the nearly complete nitrification.