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Updated: Feb 3, 2026

Diffuse Optical Spectroscopy for the Quantitative Assessment of Acute Ionizing Radiation Induced Skin Toxicity Using a Mouse Model
Published on: May 27, 2016
Quantitative diffuse reflectance spectroscopy of short-term changes in tumor oxygenation after radiation in a matched
Paola Monterroso Diaz1, Samir V Jenkins2, Kinan Alhallak1
1Department of Biomedical Engineering, University of Arkansas, Fayetteville, AR 72701, USA.
Abstract:
There is a critical need to identify patients with radiation-resistant tumors early after treatment commencement. In this study, we use diffuse reflectance spectroscopy (DRS) to investigate changes in vascular oxygenation and total hemoglobin concentration in A549 radiation-sensitive and resistant tumors treated with a clinically relevant dose fraction of 2 Gy. DRS spectra were acquired before, immediately after, 24, and 48 hours after radiation. Our data reveals a significantly higher reoxygenation (sO2) in the radiation-resistant tumors 24 and 48h after treatment, and provides promising evidence that DRS can discern between the reoxygenation trends of radiation-sensitive and resistant tumors.
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