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Virus expression. EGF and transferrin receptors in human papillomas
Virchows Archiv. A, Pathological Anatomy and Histopathology
|January 1, 1987
Summary
Human papillomavirus (HPV) infection alters EGF and transferrin receptor expression in skin and mucosal papillomas. Viral DNA, not just viral proteins, may drive cell growth, suggesting immune responses also modulate papilloma development.
Area of Science:
- Oncology
- Virology
- Immunology
Background:
- Human papillomavirus (HPV) causes various skin and mucosal lesions.
- The role of epidermal growth factor (EGF) and transferrin receptors in papilloma development is not fully understood.
Purpose of the Study:
- To investigate the expression of EGF and transferrin receptors in cutaneous and mucosal papillomas.
- To correlate receptor expression with the presence of HPV DNA, viral antigens, and host immune responses.
Main Methods:
- Indirect immunofluorescence was used to detect EGF and transferrin receptors on frozen papilloma sections.
- In situ hybridization with biotinylated probes identified HPV DNA.
- Viral capsid antigen and DR antigen expression were also examined.
Main Results:
- EGF and transferrin receptor expression patterns were altered in lesions with significant viral cytopathic effects.
- Receptor expression was enhanced in mucosal lesions, particularly laryngeal papillomas, which are less efficient virus producers.
- Increased receptor reactivity correlated with higher detection of HPV DNA compared to viral antigen.
- DR antigen expression was observed in epithelial cells of some lesions with inflammatory reactions.
Conclusions:
- HPV DNA may contribute to basal cell stimulation in papillomas.
- Epithelial cell activation in papillomas can be influenced by factors beyond HPV, including mediators of the local immune response.