Related Experiment Video

Updated: Feb 3, 2026

Visualization of Vascular Ca2+ Signaling Triggered by Paracrine Derived ROS
07:33

Visualization of Vascular Ca2+ Signaling Triggered by Paracrine Derived ROS

Published on: December 21, 2011

16.0K

Protective effects ROS up-regulation on premature ovarian failure by suppressing ROS-TERT signal pathway

H-L Jiang1, L-Q Cao, H-Y Chen

  • 1Department of Obstetrics, The Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China. ganyapingya@163.com.

European Review for Medical and Pharmacological Sciences
|October 20, 2018
PubMed
Abstract

Insights

Reactive oxygen species (ROS) elevate in premature ovarian failure (POF), inhibiting telomerase reverse transcriptase (TERT) and worsening the condition. Targeting the ROS-TERT pathway may offer a new treatment for POF.

Area of Science:

  • Gynecology
  • Reproductive Biology
  • Cellular Biology

Background:

  • Premature ovarian failure (POF) is a gynecological condition with unclear pathogenic mechanisms.
  • Decreased telomerase reverse transcriptase (TERT) activity has been observed in POF.
  • The role of oxidative stress, a suppressor of TERT, in POF remains understudied.

Purpose of the Study:

  • To investigate the involvement of reactive oxygen species (ROS) and TERT in the pathogenesis of POF.
  • To explore the potential of targeting the ROS-TERT pathway for POF treatment.

Main Methods:

  • A rat model of POF was established using cyclophosphamide and busulfan.
  • Hormone levels (FSH, inhibin B) and ovarian histology were assessed.
  • Western blot was used to measure TERT expression.
  • N-acetyl-cysteine (NAC) was used to reduce ROS, and TERT small interfering RNA (siRNA) was used to suppress TERT.

Main Results:

  • POF models exhibited ovarian atrophy, reduced follicles, increased follicular atresia, and elevated FSH and decreased inhibin B levels.
  • Elevated ROS and decreased TERT levels were observed in POF rats.
  • NAC treatment reduced ROS and increased TERT expression, ameliorating POF.
  • TERT siRNA exacerbated the POF condition.

Conclusions:

  • Increased ROS inhibits TERT expression and activity, contributing to POF.
  • The ROS-TERT pathway is implicated in POF pathogenesis.
  • Modulating the ROS-TERT pathway presents a potential therapeutic strategy for POF.

More Related Videos

ROS Live Cell Imaging During Neuronal Development09:25

ROS Live Cell Imaging During Neuronal Development

Published on: February 9, 2021

4.8K
Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking13:20

Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking

Published on: March 7, 2019

16.6K

Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
6.6K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
10.1K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.4K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
8.5K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
10.6K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
10.0K