Predation Risk Potentiates Toxicity of a Common Metal Contaminant in a Coastal Copepod

To examine whether natural stressors like predation risk affect responses to anthropogenic contaminants, we exposed nauplii of the copepod Tigriopus brevicornis to chemical cues from fish (kairomones) and copper (Cu). We tested effects of these treatments, singly and combined, on copepod age and size at maturity, and development stage sensitivity, while controlling for effects of genetic heterogeneity (clutch identity). Predation risk, Cu and clutch identity interacted in their effect on development time. Predation risk alone had minor effects, but potentiated Cu toxicity in the combined treatment by doubling the delay in age at maturity, as compared to Cu exposure alone. This potentiating effect on developmental delay appeared already at the first copepodite stage. The specific strength of response varied among nauplii from different females' clutches. There were no differences in copepod size at maturity among treatments. We did, however, find an interaction between the effect of Cu and clutch identity on copepod growth. Our results demonstrate the importance of ecological interactions for potentiating the toxicity of environmental contaminants. We also demonstrate the need to consider genetic heterogeneity in ecotoxicology. Natural variation in stressor responses has implications for the interpretation of results from toxicological studies using single-clone or inbred culture populations.