Related Experiment Video

Updated: Feb 3, 2026

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
09:45

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

Published on: January 1, 2017

11.4K

Microglia Are Derived from Peripheral Blood Mononuclear Cells After Pseudorabies Infection in Mice

Shouping Hu1, Qiang Liu1, Sufang Zang1

  • 1National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute , Chinese Academy of Agricultural Sciences, Harbin, China .

Viral Immunology
|October 20, 2018
PubMed

Insights

Pseudorabies virus (PRV) infects the central nervous system, causing brain lesions and increasing microglia. PRV activates microglia, but new microglia originate from blood monocytes, not local proliferation.

Area of Science:

  • Veterinary Neurology
  • Immunology
  • Virology

Background:

  • Pseudorabies virus (PRV) invades the central nervous system via peripheral nerves, causing brain lesions.
  • Microglia play a crucial role in the central nervous system's response to viral infections.

Purpose of the Study:

  • To investigate microglial dynamics, recruitment, and polarization following PRV inoculation.
  • To understand the interaction between PRV and microglia in a mouse model.

Main Methods:

  • Subcutaneous inoculation of mice with PRV-Bartha K61 vaccine strain at varying doses.
  • Analysis using immunohistochemistry, flow cytometry, and confocal laser scanning microscopy.
  • 5-bromo-deoxy-uridine (BrdU) labeling to track cell proliferation.

Main Results:

  • Increased mortality and brain lesions (brain tail, brain stem) correlated with PRV dose.
  • PRV inoculation induced microglial activation and proliferation markers (Pcna, Iba1).
  • BrdU labeling revealed proliferating cells were blood monocytes, not in situ microglia; microglia polarized to M1/M2 phenotypes.

Conclusions:

  • PRV triggers microglial activation and recruitment of peripheral monocytes to the brain.
  • Understanding these microglial dynamics can inform improved treatment strategies for PRV infections.
Keywords:
cell proliferationinteractionmicrogliapolarizationpseudorabies virus

More Related Videos

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells12:52

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Published on: November 28, 2015

16.5K
Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells05:38

Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells

Published on: February 12, 2021

4.7K

Related Concept Videos

Protein Buffers in Blood Plasma and Cells01:20

Protein Buffers in Blood Plasma and Cells

The human body utilizes protein buffer systems to maintain a stable pH. These systems capitalize on the dual role of amino acids, which can act as acids or bases by accepting or releasing hydrogen ions in response to pH changes. Protein buffer systems are particularly significant in the extracellular fluid (ECF) and intracellular fluid (ICF) of active cells, where structural and functional proteins provide substantial buffering capacity.
Certain amino acids can exist in a zwitterion state at a...
3.8K
Measurement: Derived Units03:02

Measurement: Derived Units

The International System of Units or SI system, by international agreement, has fixed measurement units for seven fundamental properties: length, mass, time, temperature, electric current, amount of substance, and luminosity. These are called the SI base units.
55.5K
Infection01:20

Infection

When a pathogen enters the body and reproduces, it can cause an infection, damage body cells, and cause illness symptoms that eventually lead to disease. Therefore, its prevention requires breaking the chain of infection.
The chain begins with pathogens: bacteria, viruses, fungi, prions, or parasites such as protozoa helminths. These can be present on the skin as transient or resident flora, or they can be acquired from the environment. Identifying and treating the type of infection and...
12.6K
Higher Derivatives01:29

Higher Derivatives

In calculus, higher-order derivatives extend the idea of differentiation beyond the first derivative to capture successive rates of change. These derivatives provide detailed information about the behavior of functions and have important applications in both mathematics and physics. To illustrate these concepts, consider the example functionwhich serves as a useful case study for exploring higher derivatives.The first derivative represents the slope of the original function. The second...
82
Peripheral Nervous System: Ganglia and Nerves01:24

Peripheral Nervous System: Ganglia and Nerves

The Peripheral Nervous System (PNS) is a crucial component of the body's neural network, extending beyond the central nervous system (CNS) to bridge the gap between the CNS and the external environment. It encompasses nerves, ganglia, and sensory receptors.
Nerves
The nerve is a bundle of axons that serves as the communication highway in the PNS. Each nerve is ensheathed in a protective layer of connective tissue called the epineurium. This outermost layer safeguards the nerve and supports the...
5.5K
Peripheral Artery Disease I: Introduction01:30

Peripheral Artery Disease I: Introduction

Peripheral artery disease (PAD) predominantly results from atherosclerosis, which involves the accumulation of fatty deposits, or plaques, within the walls of arteries. This causes them to narrow and harden, significantly reducing blood flow. PAD predominantly affects the legs, particularly the arteries supplying the thighs and calves. In rare cases, it may involve other arteries, including those in the arms.Etiology of PAD:The principal cause of PAD is atherosclerosis, which results from fatty...
378