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Updated: Feb 3, 2026

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip
Published on: March 29, 2024
Rapid and sensitive detection of the vanA resistance gene from clinical Enterococcus faecium and Enterococcus
Qian-Qian Huang1, Bin-Bin Liu1, Hui-Fen Zhu1
1Systemomics Center, College of Pharmacy, and Genomics Research Center, Harbin Medical University, Harbin, China; HMU-UCCSM Center for Infection and Genomics, Harbin Medical University, Harbin, China; Translational Medicine Research and Cooperation Center of Northern China, Heilongjiang Academy of Medical Sciences, Heilongjiang, China.
Objectives:
Vancomycin resistance in Enterococcus spp., mediated mainly by the vanA resistance gene, has become a major health concern as it has spread worldwide. Therefore, a rapid method is urgently required to detect the vanA gene for timely and appropriate antimicrobial control of resistant Enterococcus infections.
Methods:
The loop-mediated isothermal amplification (LAMP) assay was optimised for vanA detection in Enterococcus spp. isolates.
Results:
The LAMP primer set designed in this study could reliably recognise seven distinct regions of the vanA gene and amplify the gene within 25min at an isothermal temperature of 65°C with high specificity. The sensitivity of the optimised assay was high, with a detection limit for vanA as low as 100pg/μL, which is 100-fold more sensitive than the PCR assay. A special advantage of this optimised LAMP method is that the vanA gene could be detected directly from clinical specimens.
Conclusion:
This optimised LAMP assay has great application potential for efficient detection of vanA in clinical diagnosis and epidemiological studies.
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