Tebuconazole induces triazole-resistance in Aspergillus fumigatus in liquid medium and soil

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Ning Cui1, Yiwen He1, Shijie Yao1

  • 1Institute of Pesticide and Environmental Toxicology, College of Agricultural and Biotechnology, Zhejiang University, Hangzhou 310058, China.

The Science of the Total Environment
|October 21, 2018
PubMed

Insights

Tebuconazole exposure can induce triazole resistance in Aspergillus fumigatus, a common cause of invasive aspergillosis. This resistance can emerge through specific gene mutations or overexpression, impacting antifungal treatments.

Area of Science:

  • Medical Mycology
  • Environmental Microbiology
  • Agricultural Science

Background:

  • Aspergillus fumigatus causes invasive aspergillosis, a serious threat to immunocompromised individuals.
  • Increasing triazole resistance in A. fumigatus poses a significant global health concern.
  • Understanding resistance mechanisms is crucial for effective antifungal strategies.

Purpose of the Study:

  • To investigate the induction of triazole resistance in A. fumigatus by tebuconazole.
  • To identify genetic mutations and molecular mechanisms associated with tebuconazole-induced resistance.
  • To assess the implications of agricultural tebuconazole use on antifungal resistance.

Main Methods:

  • Exposure of A. fumigatus to tebuconazole in liquid culture and soil.
  • Isolation and characterization of resistant strains.
  • Genetic analysis of the cyp51A gene and other relevant genes (AtrF, AfuMDR1, cyp51B).
  • Assessment of hereditary stability of resistance.

Main Results:

  • Tebuconazole induced resistance in A. fumigatus with specific TR46/Y121F/T289A mutations in liquid medium.
  • Nine tebuconazole-resistant strains lacking cyp51A mutations were isolated from treated soil.
  • Two resistant isolates (HI-30, HI-36) showed overexpression of AtrF, AfuMDR1, cyp51A, and cyp51B with hereditary stability.
  • Resistance induction occurred at tebuconazole concentrations of 0.5-5.0 mg/kg in soil.

Conclusions:

  • Tebuconazole can induce triazole resistance in A. fumigatus through distinct mechanisms.
  • Agricultural application of tebuconazole may contribute to the emergence of medical triazole resistance in A. fumigatus.
  • This highlights a potential One Health issue linking agricultural practices to clinical antifungal resistance.
Keywords:
Aspergillus fumigatusFungicideResistanceTebuconazole

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